IL-2 Combined with IL-15 Enhanced the Expression of NKG2D Receptor on Patient Autologous NK Cells to Inhibit Wilms’ Tumor via MAPK Signaling Pathway

Figure 5

IL-2 and IL-15 enhance the quantity and cytotoxicity of patient autologous NK cells. Unculture and IL-2 and IL-15 costimulation culture 3 days and 7 days patient autologous CD3^-CD56⁺ NK cell in PBMC detected by FACS (a). The statistical results of CD3^-CD56⁺ NK cell in PBMC (a). Unculture and IL-2 and IL-15 costimulation culture 3 days and 7days CD3^-CD56⁺ NK cell morphology observed under a microscope, respectively (b). The statistical results of CD3^-CD56⁺ NK cell quantity in per microliter (b). Cytotoxic cytokine (CD107a and IFN-R) expression level of NK cells was detected by FACS (c). The proportion of CD107a + IFN-R NK cells (c). For comparisons, Student’s -test was performed in two group, or one-way analysis of variance (ANOVA) was performed in multiple groups. and .

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