MiRNA-196-5p Promotes Proliferation and Migration in Cholangiocarcinoma via HAND1/Wnt/β-Catenin Signaling Pathway

Figure 1

Inhibition of miR-196-5p suppressed cell proliferation and migration in CCA. (a) The intersection of genes upregulated in CCA from the GSE47764 and GSE140001 databases. (b) RT-qPCR results showed the expression of miR-196-5p increased in three different CCA cell lines compared to normal biliary epithelial cell line HIBEC. U6 was used as an internal control for miR-196-5p. Student’s t-test was used to analyze the statistical significance and all error bars signify standard deviations ( = 3). HuCCT1 was chosen for subsequent experiments for miR-196-5p showed the highest increasing rate in it. (c) RT-qPCR verified the transfection efficiency of miR-196-5p inhibitor (Inhi- miR-196-5p). (d) and (e) The effect of Inhi- miR-196-5p on the proliferation capacity of HuCCT1 in vitro was detected using EdU (d [left]), colony formation (d [right]), and CCK-8 assay (e). There is a statistical significance between the EdU positive cell percentage in the control group compared to in Inhi-miR-196-5p treated group (). Scale bars, 100 μm. (f) and (g) Migration as well as matrigel invasion assay was performed to detect the effect of miR-196-5p depletion on cell migration and invasion in HuCCT1. There is a statistical significance between the migrated cell count in the control group compared to in Inhi-miR-196-5p treated group (). There is a statistical significance between the invasion cell count in the control group compared to in Inhi-miR-196-5p treated group (). Scale bars, 150 μm.

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