MiRNA-196-5p Promotes Proliferation and Migration in Cholangiocarcinoma via HAND1/Wnt/β-Catenin Signaling Pathway

Figure 2

HAND1 is identified as the targeting mRNA of miR-196-5p. (a) Venn diagram depicting the intersection between the miR-196-5p and predictor targeting mRNA. (b) HeLa cells were co-transfected with wild-type plasmid (WT-HAND1) with miR-196-5p or NC and treated mutant plasmid (Mut-HAND1) with miR-196-5p or NC. Luciferase activity was measured 48 hours after transfection. . (c) After treatment with miR-196-5p and NC for 48 hours, the relative expression level of HAND1 was detected by RT-qPCR. There is a statistical significance between the expression level of miR-196-5p in the control group compared to mimic-miR-196-5p treated group ().

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