MiRNA-196-5p Promotes Proliferation and Migration in Cholangiocarcinoma via HAND1/Wnt/β-Catenin Signaling Pathway

Figure 3

miR-196-5p promotes proliferation and migration of HuCCT1 in a HAND1 and activation manner. (a) and (b) The effect of HAND1 or β-catenin depletion on the proliferation capacity of HuCCT1 in vitro was detected using EdU (a [upper]), colony formation (a [lower]), and CCK-8 assay (b). The downregulated cell proliferation of HuCCT1 was reversed by HAND1 knockdown or β-catenin silencing. HAND1 OE: HAND1 overexpression; HAND1 KD: HAND1 knockdown. There is a statistical significance between the EdU positive cell percentage in blue, compared to red or green group. Scale bars, 100 μm. (c) and (d) Migration and matrigel invasion assays were performed to detect the effect of HAND1 or β-catenin depletion on cell migration and invasion in HuCCT1. There is a statistical significance between the EdU positive cell percentage in blue, compared to red or green group. Scale bars, 150 μm. Blue panel: Inhi-miR-196-5p, red panel: Inhi-miR-196-5p + HAND1 KD, green panel: Inhi-miR-196-5p+si-β-catenin, purple panel: HAND1 OE.

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