MiRNA-196-5p Promotes Proliferation and Migration in Cholangiocarcinoma via HAND1/Wnt/β-Catenin Signaling Pathway

Figure 4

miR-196-5p/HAND1/β-catenin axis regulates cell proliferation and EMT process in HuCCT1. (a) Western blot of protein expression of HAND1, proliferation maker Ki67, E-cadherin, and N-cadherin in HuCCT1 with representative images of protein bands for each panel. (b) HuCCT1 was stained with anti-β-catenin antibody (red) and DAPI (blue). Pink color indicates the overlap of red and blue colors. White arrows point to the translocation of β-catenin expression from membrane to nuclear, which indicates the activation of Wnt/β-catenin signaling pathway. Scale bars, 10 μm.

(a)

(b)