Research Article
MiRNA-196-5p Promotes Proliferation and Migration in Cholangiocarcinoma via HAND1/Wnt/β-Catenin Signaling Pathway
Table 2
The plasmid sequence of Mimic-NC should be GTTUTCCGAACGTGTCACGT.
(a) |
| | Gene symbol | |
| | Plasmid | Sequence (5-3) | | Inhi-NC | CAGUACUUUUGUGUAGUACAA | | Inhi-miR-196-5p | CCCAACAACAGGAAACTACCTA | | Mimic-NC | GTTVTCCGAACGTGTCACGT | | Mimic- miR-196-5p | TAGGTAGTTTCCTGTTGTTGGG |
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(b) |
| | Primer | Forward | Reverse |
| | HAND1 | AAAGGCCCTACTTCCAGAGC | GTCCATCAGGTAGGCGATGT | | U6 | GCTTCGGCAGCACATATACT | GAGCAGGCTGGAGAA | | GAPDH | ACAACTTTGGTATCGTGGAAGG | GCCATCACGCCACAGTTTC |
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