Glycoprotein M6A Suppresses Lung Adenocarcinoma Progression via Inhibition of the PI3K/AKT Pathway

Figure 2

GPM6A suppression promotes the growth and migration of lung adenocarcinoma cells. (a) GPM6A mRNA expression in A549 and H1299 cells transfected with si-NC and si-GPM6A-1 and -2. (b) GPM6A protein expression in A549 and H1299 cells transfected with si-NC and si-GPM6A-1 and -2. (c) OD value (450 nm) of cells at 0, 24, 48, and 72 h of transfection with si-NC and si-GPM6A-1 and -2. (d) Relative number of EdU-positive cells after transfection with si-NC and si-GPM6A-1 and -2. (e) Colony-forming numbers of cells after transfection with si-NC and si-GPM6A-1 and -2. (f) Migration area of cells after transfection with si-NC and si-GPM6A-1 and -2. (g) Number of migrating cells after transfection with si-NC and si-GPM6A-1 and -2. (h) Immunofluorescence expression of EMT markers (E-cadherin, N-cadherin, and vimentin) after transfection with si-NC and si-GPM6A-1 and -2. (i) The expression of EMT markers (E-cadherin, N-cadherin, Zeb1, vimentin, and snail) on WB after transfection with si-NC and si-GPM6A-1 and -2. (j) Expression of EMT markers (E-cadherin, N-cadherin, Zeb1, vimentin, and snail) on RT-qPCR after transfection with si-NC and si-GPM6A-1 and -2. Data shown are the mean ± standard deviation (SD) for three independent experiments (, , , and ) (OD: optical density and EMT: epithelial-mesenchymal transition).

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