Aspirin Suppressed PD-L1 Expression through Suppressing KAT5 and Subsequently Inhibited PD-1 and PD-L1 Signaling to Attenuate OC Development

Figure 4

Aspirin stimulates T cells proliferation and activation to inhibit OC cell survival. (a–e) OC cells were cocultured with unstimulated or activated T cells in Transwell. (a) The level of PD-L1 in cells was checked by FACS. (b and c) Flow cytometry to check the portion of CD8⁺ PD-1⁺ and CD4⁺ PD-1⁺ T cell. (d) Level of PD-L1 in TOV-21G and SKOV-3 cells incubated with conditioned medium collected from unstimulated or activated PBMCs. (e) CCK-8 assay to detect proliferation of T cells in the coculture system. (f) Flow cytometry to determine the portion of CD8+ T cells in the coculture system. (g) Flow cytometry to measure T cell apoptosis. .

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