Research Article
LIMS2 is Downregulated in Osteosarcoma and Inhibits Cell Growth and Migration
Figure 5
LIMS2 expression was declined in OS cells. (a) qRT-PCR and (b,cC) western blotting assays were applied to assess the mRNA and protein levels of LIMS2 in normal hFOB 1.19 cells and OS cell lines (U-2OS, MG-63, Saos-2, and MNNG/HOS). (d) MS-PCR was applied to detect the methylation levels of LIMS2 in hFOB 1.19 cells and OS cell lines (U-2OS, MG-63, Saos-2, and MNNG/HOS) (, , and ).
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