Hypoxia Confers Tumor with a Higher Immune Infiltration but Lower Mutation Burden in Gastrointestinal Cancer

Figure 3

Hypoxic gastrointestinal tumors with low TMB may also have high immune infiltration. (a) Relatively lower TMB was found in the hypoxic group than in the normoxic group. (b) The number of SNV-derived neoantigens was much larger in the hypermutated tumors, irrespective of hypoxic status. (c) The number of INDEL-derived neoantigens was much larger in the hypermutated tumors, irrespective of hypoxic status. (d) The infiltration of stromal cells were much more abundant in the hypoxic group than that in the normoxic group, irrespective of mutation status. (e) The infiltration of immune cells were subjected to regulation by both mutation and oxygen content. (f) Survival differences stratified by the hypoxic status and TMB revealed that the hypoxic-hypermutated group had no survival difference compared to the hypoxic-nonhypermutated group but displayed a worse prognosis than the normoxic-hypermutated and normoxic-nonhypermutated groups in gastrointestinal cancer. (g) Flow cytometry was used to monitor the efficiency of isolation of CD8 T cells. (h) CD8 T cells had higher cytotoxicity than tumor cells under normoxic conditions in vitro as evaluated by the T cell cytotoxicity assay.

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