WDR72 Enhances the Stemness of Lung Cancer Cells by Activating the AKT/HIF-1α Signaling Pathway

Figure 3

Knockdown of WDR72 inhibits LCSC proliferation and migration, and promotes apoptosis. (a) The viability of LCSCs detected by CCK-8 assay (b) The proliferation of LCSCs detected by colony formation assay. (c) The changes in colony number. (d) and (e) Abundance of proliferation-associated proteins. (f) and (g) The migration of LCSCs detected by Transwell assay. (h) and (i) Abundance of EMT-associated proteins. (j) and (k) The apoptosis of LCSCs analyzed by flow cytometry. (l) and (m) Abundance of apoptosis-associated proteins.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)

(m)