circRNA TCFL5 Promote Esophageal Cancer Progression by Modulating M2 Macrophage Polarization via the miR-543-FMNL2 Axis

Figure 4

FMNL2 was a downstream target gene of miR-543. (a) TargetScan was used to predict the targets of miR-543 and a binding site on position 1596-1603 at the 3UTR of FMNL2. (b) Dual-luciferase reporter gene assay was used to detect the binding between miR-543 and FMNL2 in Eca109 cells. , vs. NC group. (c, d) qPCR and western blot analysis were used to detect the expression of FMNL2 in esophageal cancer tissues, vs. control group. (e) The expression of FMNL2 in Eca109 cells affected by miR-543 was detected by western blot. Data are shown as the , , miR-543 mimics vs. NC group; ^##, miR-543 inhibitor vs. NC group.

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