Tumour: Fibroblast Interactions Promote Invadopodia-Mediated Migration and Invasion in Oral Squamous Cell Carcinoma

Figure 1

Indirect culture of oral squamous cell carcinoma (OSCC) cells with normal oral fibroblasts and cancer-associated fibroblasts (CAFs) promotes cancer cell migration and invasion. H357 and SCC4 cells were incubated (24 h) in conditioned medium (CM) collected from normal oral fibroblasts (NOFs), experimentally induced CAFs, or serum-free medium (SFM). (a) Images of cancer cells were captured by light microscopy. (b), (c) Cell migration was assessed by a Transwell system. (d), (e) Cell invasion through matrigel was examined. Representative images of migrated and invaded cells are shown under the respective experimental conditions, and the cells were quantified by counting cells on 10 randomly selected images. All experiments were conducted three times. The data are mean ± standard deviation (SD), ^∗, ^∗∗, ^∗∗∗.

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