Circular RNA circ_0004488 Increases Cervical Cancer Paclitaxel Resistance via the miR-136/MEX3C Signaling Pathway

Figure 1

Increased circ_0004488 levels in CSC-enriched populations of cervical cancer cells. (a) QRT-PCR analysis showed that CSC-enriched spheres had a higher concentration of CSC markers compared to parental cell lines. (b) PRPSAP2 exons are back-spliced to create circ_0004488. (c) Expression of circ_0004488 was examined in cervical cancer cell lines and a normal cell line using quantitative real-time polymerase chain reaction. (d) Expression of circ_0004488 and PRPSAP2 mRNA was measured in cervical cancer cells treated with or without RNase R by quantitative real-time polymerase chain reaction. (e) Circ_0004488 expression in human cervical cancer and adjacent normal tissues. (f) Circ_0004488 expression in either paclitaxel-sensitive or -resistant SiHa cells. (g) Circ_0004488 tissue levels in paclitaxel-resistant and -sensitive cervical carcinoma. (h) A comparison of the prognosis for individuals with cervical cancer who express high levels of circ_0004488 with those who express low levels of this gene is shown in the panel. (i) Circ_0004488 was mostly located in the cytoplasm of cervical cancer cells. .

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