Circular RNA circ_0004488 Increases Cervical Cancer Paclitaxel Resistance via the miR-136/MEX3C Signaling Pathway

Figure 3

Circ_0004488 binds to miR-136 and acts as a molecular sponge for miR-136. (a) Circ_0004488 and miR-136 were found to potentially interact in the CircInteractome database. (b) The difference in survival rates between patients with high and low miR-136 expression in cervical cancer. (c) The circ_0004488 biotinylated probe and a control probe were transfected into cervical cancer cells. Circ_0004488 expression was quantified by qRT-PCR after streptavidin capture. As a negative control, we employed miR-1248. (d) MiR-136 expression in miR-136 mimic or inhibitor transfected cervical cancer cells. (e) Cervical cancer cells were transfected with miR-136 mimics (or miR-136 inhibitor) and either wild-type (WT) or mutant (MUT) circ_0004488 luciferase reporter vectors, and the relative luciferase activity was evaluated. (f) qRT-PCR experiments were used to analyze miR-136 expression in cervical cancer cells treated with a circ_0004488 vector or circ_0004488 siRNA. (g) In order to determine circ_0004488 and miR-136 expression in cervical cancer cells, the Ago2 RIP assay was performed. A qRT-PCR test was used to analyze circ_0004488 and miR-136 expression. .

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