Sirtuin 4 Inhibits Prostate Cancer Progression and Metastasis by Modulating p21 Nuclear Translocation and Glutamate Dehydrogenase 1 ADP-Ribosylation

Figure 2

SIRT4 inhibits proliferation and cell cycle in PCa cells. (a) The SIRT4 protein expression levels in SIRT4-overexpressing PC-3 (left) and SIRT4-knockdown 22rv1 (right) cells were detected by western blotting. (b) CCK8 assay. The effect of SIRT4 overexpression or silencing on the proliferation of PC-3 and 22rv1. (c) Colony formation assay SIRT4-overexpressing cells and SIRT4-silenced cells were seeded onto 6-plates, and the number of colonies was counted on day 14. (d) EdU assay was used to detect proliferation ability in PC-3 and 22RV1 cells. (e) Wound-heal assay was detected at 0 h and 24 h to determine cell migration ability in SIRT4-overexpressing and SIRT4-knockdown PCa cells. (f) Transwell assays were used to determine the invasion and migration abilities of SIRT4-overexpressing and SIRT4-knockdown PCa cells. Scale bar = 100 μm , .

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