Metformin Exhibits an Attractive Antineoplastic Effect on Human Endometrial Cancer by Regulating the Hippo Signaling Pathway

Figure 1

Metformin inhibited cell proliferation and induced cell cycle arrest in endometrial carcinoma cells. Ishikawa and RL95-2 cells were incubated with metformin at different concentrations (0, 5, 10, 20, and 40 mM for Ishikawa cells and 0, 5, 10, and 20 mM for RL95-2 cells), and the cell proliferation ability was detected using CCK-8 assays. (a and b) Cell proliferation inhibition of Ishikawa cells was dependent on the dose and time of metformin exposure. (c and d) Metformin mediated RL95-2 cell growth inhibition in a dose- and time-dependent manner. (e) Compared with the control group (g), 10 mM (h) and 20 mM (i) metformin treatment resulted in cell cycle arrest at the G1 phase in Ishikawa cells. (f) 8 mM (k) metformin was capable of arresting the cell cycle at the G2 phase in PL95-2 cells in comparison with the nontreated group (j). Each experiment was performed in triplicate and repeated at least three times independently. Data are presented as the mean ± SD. ; ; versus controls. Met, metformin; Con, control.

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