Metformin Exhibits an Attractive Antineoplastic Effect on Human Endometrial Cancer by Regulating the Hippo Signaling Pathway

Figure 3

Metformin inhibited cell migration and invasion in endometrial carcinoma cells. Cell migration ability was detected using a wound healing assay and transwell migration assay. The invasion ability was determined using in vitro transwell invasion assays. (a and b) The decrease in relative areas between the scratches in the higher concentration metformin treatment group was less evident, which indicated an inhibitory effect of metformin on Ishikawa cell migration. (c and d) Metformin greatly inhibited cell migration in Ishikawa cells. (e and f) The inhibition of cell migration in metformin-treated RL95-2 cells was more significant. (g and h) Cell invasion after exposure to different concentrations of metformin in Ishikawa cells. (i and j) Cell invasion was inhibited by metformin in RL95-2 cells. Each experiment was performed in triplicate and repeated at least three times independently. Data are presented as the mean ± SD. ; versus controls. Met, metformin; Con, control.

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