MiR-522-3p Targets Transcription Factor 4 to Overcome Cisplatin Resistance of Gastric Cells

Figure 3

miR-522-3p targeted TCF4 in GC cells. (a) RT-qPCR measured TCF4 mRNA level in AGS cells under NC mimics or miR-522-3p mimics transfection.  < 0.05, miR-522-3p vs. NC mimics group. (b) Western blotting detected miR-522-3p protein level in AGS cells under NC mimics or miR-522-3p mimics transfection. (c) starBase predicted binding fragment of miR-522-3p on TCF4 3′UTR. (d) Luciferase reporter assay assessed relationship of miR-522-3p and TCF4 in GC cells.  < 0.05, miR-522-3p vs. NC mimics group. (e) RT-qPCR measured TCF4 level in control cells and GC cell lines.  < 0.001, AGS, HGC27, MKN-45 vs. GES-1 group. (f) RT-qPCR detected TCF4 level in AGS cells under DDP treatment at different doses.

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