CAPRIN1 Enhances Chemoresistance and Glycolysis in Laryngeal Squamous Cell Carcinoma via Regulation of ZIC5

Figure 2

Forced expression of CAPRIN1 promoted glycolysis and chemoresistance in laryngeal cancer cells. (a and b) qRT-PCR and Western blotting were used to determine the mRNA and protein expression levels of CAPRIN1, respectively, in Hep-2 cells that were transfected with CAPRIN1 plasmid (pSin-CAPRIN1) or empty vector (pSin-vec). (c and d) The cellular uptake of 2-DOG and lactate secretion were also determined in those transfected Hep-2 cells. (e and f) qRT-PCR and Western blotting were used to determine the mRNA and protein expression levels of multidrug transporter expression (ABCG2 and ABCB1), respectively, in Hep-2 cells that were transfected with CAPRIN1 plasmid (pSin-CAPRIN1) or empty vector (pSin-vec). (g and h) Cell viability and colony formation were determined to evaluate cell viabilities in cisplatin-treated Hep-2 cells that were transfected with CAPRIN1 plasmid (pSin-CAPRIN1) or empty vector (pSin-vec). The data were shown as the . , , and .

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