CAPRIN1 Enhances Chemoresistance and Glycolysis in Laryngeal Squamous Cell Carcinoma via Regulation of ZIC5

Figure 3

Knockdown of CAPRIN1 inhibited glycolysis and chemoresistance in laryngeal cancer cells. (a and b) qRT-PCR and Western blotting were used to determine the mRNA and protein expression levels of CAPRIN1, respectively, in TU-177 and TU-177/R cells that were transfected with CAPRIN1 siRNAs (si-CAPRIN1#1 and si-CAPRIN1#2) or negative control (si-NC). (c and d) Besides, the cellular uptake of 2-DOG and lactate secretion were also determined. (e and f) qRT-PCR and Western blotting were used to determine the mRNA and protein expression levels of multidrug transporter expression (ABCG2 and ABCB1), respectively, in TU-177/R cells that were transfected with CAPRIN1 siRNAs (si-CAPRIN1#1 and si-CAPRIN1#2) or negative control (si-NC). (g and h) Cell viability and colony formation assay were determined in cisplatin-treated TU-177/R cells that were transfected with CAPRIN1 siRNAs (si-CAPRIN1#1 and si-CAPRIN1#2) or negative control (si-NC). The data were shown as the . , , and .

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