CAPRIN1 Enhances Chemoresistance and Glycolysis in Laryngeal Squamous Cell Carcinoma via Regulation of ZIC5

Figure 4

CAPRIN1 regulated the ZIC5 expression. (a and b) qRT-PCR was used to determine the mRNA expression levels of ZIC5 in Hep-2 cells that were transfected with CAPRIN1 plasmid (pSin-CAPRIN1) or empty vector (pSin-vec) and mRNA expression levels of ZIC5 in TU-177 cells that were transfected with CAPRIN1 siRNAs (si-CAPRIN1#1 and si-CAPRIN1#2) or negative control (si-NC). In addition, (c and d) after the cells were transfected for 48 h, the cells were then treated with actinomycin D (10 μg/ml). These cells were harvested at 0, 2, 4, 6, and 8 h after treatment. The mRNA expression levels of ZIC5 were then detected using qRT-PCR. The data were shown as the . and .

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