CAPRIN1 Enhances Chemoresistance and Glycolysis in Laryngeal Squamous Cell Carcinoma via Regulation of ZIC5

Figure 5

CAPRIN1 regulated ZIC5 through 3UTR binding. (a) A biotinylated RNA pull-down assay was performed. The biotinylated different regions of ZIC5 mRNA were incubated with cell lysates (Hep-2 and TU-177) and the mRNA region that was interacted with CAPRIN1 was analyzed by using Western blotting. β-Actin was used as an internal control. Besides, (b) RNP IP assay was also operated. After mRNA of ZIC5 in cell lysates were captured by anti-CAPRIN1 antibody or anti-IgG, qRT-PCR was then determined. (c) Luciferase reporter activity was performed in the Hep-2 cells that were cotransfected with pSin-CAPRIN1/pSin-vec and ZIC5 3UTR/psi-check2, respectively. (d) Luciferase reporter activity was detected in the TU-177 cells that were cotransfected with si-CAPRIN1#1/si-CAPRIN1#2/si-NC and ZIC5 3UTR/psi-check2, respectively.

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