MiR-300 Alleviates Cell Proliferation and Migration and Facilitates Cell Apoptosis by Targeting c-Met in Gastric Cancer

Figure 3

c-Met is a direct target gene of miR-300 in GC. (a) Schematic description of the predicted binding site of miR-300 in the 3UTR of c-Met gene. (b–d) qRT-PCR analysis of miR-300 levels in MKN-45, SGC-7901, and AGS cells after transfection with miR-300 mimic or inhibitor. (e and f) WB analysis of c-Met protein levels in 3 GC cell lines after transfection with miR-300 mimic or inhibitor. (g) Relative luciferase activities in MKN-45 after transfection with miR-300 mimic or inhibitor. (h) qRT-PCR analysis of miR-300 levels in GC tissue pairs. (i) Pearson analysis of the relationship between miR-300 level and c-Met protein level in GC tissue pairs. , ; ; .

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