Lysosomal-Associated Transmembrane Protein 5 Promotes Proliferation, Migration, and Invasion of Clear Cell Renal Cell Carcinoma

Figure 2

LAPTM5 promotes ccRCC proliferation in vitro. (a) Western blot analysis of LAPTM5 expression in 786-0 and Caki-1 cells using a FLAG antibody. (b) Colony formation of the LAPTM5-overexpression or control groups in 786-0 and CAKI-1 cell lines. (c) EdU assay results of the LAPTM5-overexpression group or control group. Scale bar, 200 μm. (d) The expression of proliferation molecular markers. (e) Western blot analysis of LAPTM5 expression in 786-0 and Caki-1 using the LAPTM5 antibody. (f) Colony formation of the shLAPTM5 and shRNA groups. (g) EdU assay results of the shLAPTM5 or shRNA groups. Scale bar, 200 μm. (h) Western blotting results of proliferation markers in shLAPTM5 and shRNA groups. vs. control or shRNA group. Abbreviations: LAPTM5, lysosomal-associated transmembrane protein 5; ccRCC, clear cell renal clear cell carcinoma; LAPTM5-OE: LAPTM5 overexpression; shLAPTM5: short-hairpin LAPTM5; shRNA, short-hairpin RNA; PCNA: proliferating cell nuclear antigen.

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