Lysosomal-Associated Transmembrane Protein 5 Promotes Proliferation, Migration, and Invasion of Clear Cell Renal Cell Carcinoma

Figure 3

LAPTM5 promotes ccRCC cell migration and invasion in vitro. (a) Results of the wound-healing assay of the LAPTM5-overexpression or control groups in both 786-0 and Caki-1 cells. (b) Results of the wound-healing assay of the shLAPTM5 or shRNA groups in 786-0 and Caki-1 cells. (c) Migration of 786-0/Caki-1 LAPTM5-overexpression or 786-0/Caki-1 control groups. Scale bar, 100 μm. (d) Invasion of 786-0/Caki-1 LAPTM5-overexpression or 786-0/Caki-1 control groups. Scale bar, 100 μm. (e) Protein expression of migration- and invasion-associated molecular makers in 786-0/Caki-1 LAPTM5-overexpression or 786-0/Caki-1 control groups. (f)–(h) Migration, invasion, and expression of migration- and invasion-associated markers in 786-0/Caki-1 shLAPTM5 or 786-0/Caki-1 shRNA groups. Scale bar, 100 μm. vs. control or shRNA group. Abbreviations: LAPTM5, lysosomal-associated transmembrane protein 5; ccRCC, clear cell renal cell carcinoma; LAPTM5-OE: LAPTM5 overexpression; shLAPTM5: short-hairpin LAPTM5; shRNA, short-hairpin RNA.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)