Lysosomal-Associated Transmembrane Protein 5 Promotes Proliferation, Migration, and Invasion of Clear Cell Renal Cell Carcinoma

Figure 5

LAPTM5 promotes activation of the JNK/p38 axis. (a) Protein expression of phosphorylated and total MAPK in the 786-0-LAPTM5-overexpression and 786-0-control groups. (b) Protein expression of phosphorylated and total MAPK in 786-0-shLAPTM5 and 786-0-shRNA groups. (c) Protein expression of phosphorylated and total MAPK in the Caki-1-LAPTM5-overexpression and Caki-1-control groups. (d) Protein expression of phosphorylated and total MAPK in 786-0-LAPTM5-overexpression injected mice and 786-0-control injected mice. (e) and (f) Interaction between LAPTM5 and RAC1 in 293T cells. (g) and (h) Interaction between LAPTM5 and RAC1 in vitro. vs. control or shRNA groups. n = 3 independent experiments. Abbreviations: LAPTM5, lysosomal-associated transmembrane protein 5; shRNA, short-hairpin RNA; RAC1, Ras-related C3 botulinum toxin substrate 1; FLAG-LAPTM5: LAPTM5 and FLAG fusion protein; HA-LAPTM5: LAPTM5 and HA fusion protein; FLAG-RAC1: RAC1 and FLAG fusion protein; HA-RAC1: RAC1 and HA fusion protein.

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