Role of the Long Intergenic Non-Protein-Coding RNA 1278/miR-185-5p/Cystatin SN Axis in Laryngeal Cancer Cells

<div>miR-185-5p binds to CST1 3<svg height="11.4859pt" id="M27" style="vertical-align:-0.04979992pt" version="1.1" viewbox="-0.0498162 -11.4361 3.89517 11.4859" width="3.89517pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M310 541L304 571C290 586 211 619 185 610L80 76L131 52L310 541Z"></path></g></svg>UTR and suppresses its expression. (a) Expression of miR-185-5p in TU686, M4E, TU177, TU138, and control HuLa-PC cells, as analyzed by RT-qPCR. (b) Expression of miR-185-5p in the nuclear and cytoplasmatic parts of TU686 and TU177 cells, as assessed by RT-qPCR. (c) Overexpression efficiency of miR-185-5p was assessed by RT-qPCR. (d) CST1 expression in miR-185-5p mimic-transfected TU686 and TU177 cells was evaluated by RT-qPCR. (e) Sequences of miR-185-5p and the complementary binding site on CST1 3<svg height="11.4859pt" id="M28" style="vertical-align:-0.04979992pt" version="1.1" viewbox="-0.0498162 -11.4361 3.89517 11.4859" width="3.89517pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M310 541L304 571C290 586 211 619 185 610L80 76L131 52L310 541Z"></path></g></svg>UTR. (f) Luciferase activity of pmirGLO-CST1 3<svg height="11.4859pt" id="M29" style="vertical-align:-0.04979992pt" version="1.1" viewbox="-0.0498162 -11.4361 3.89517 11.4859" width="3.89517pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M310 541L304 571C290 586 211 619 185 610L80 76L131 52L310 541Z"></path></g></svg>UTR-Wt/Mut in miR-185-5p mimic-transfected TU686 and TU177 cells. <span class="nowrap"><svg height="10.7395pt" id="M30" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 -10.1025 50.9184 10.7395" width="50.9184pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 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Journal of Oncology

fig3

Figure 3

Figure 3: Role of the Long Intergenic Non-Protein-Coding RNA 1278/miR-185-5p/Cystatin SN Axis in Laryngeal Cancer Cells 