miRNA-331-3p Affects the Proliferation, Metastasis, and Invasion of Osteosarcoma through SOCS1/JAK2/STAT3

Figure 3

SOCS1 is a direct target of miR-331-3p and is considered an oncogene in OS. (a) Screening target genes through the intersection of three databases (TargetScan, miRDB, and miRTarBase). (b) qRT-PCR is used to evaluate the expression level of potential target genes in OS cells. (c) Schematic diagram of the binding sequence between miR-331-3p and SOCS1. (d) HEK-293T cells are transfected with miR-331-3p mimic or negative control (NC) and wild-type or mutant SOCS1³′-UTR, and subjected to luciferase assay. (e) qRT-PCR is used to evaluate the mRNA level of SOCS1 in OS cells transfected with miR-331-3p mimics/inhibitors. (f) Western blotting is used to evaluate the protein expression of SOCS1 in OS cells transfected with miR-331-3p mimics/inhibitors. The results are shown as the mean ± standard deviation (SD) from three independent experiments. ^∗∗.

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