Antitumor Effects of Natural Bioactive Ursolic Acid in Embryonic Cancer Stem Cells

<div>UA produces the formation of cellular and mitochondrial ROS in embryonic CSCs. (a) Immunoblotting analysis of iNOS expression in NTERA-2 and NCCIT cells after treatment for 10 or 20 <i>μ</i>M UA 24 h. Exposure rates were measured by densitometry and are standardized in <i>β</i>-actin levels. (b) Real-time qPCR analysis showed a symbolic representation of iNOS mRNA in NCCIT cells and NTERA-2 cells. The CP values obtained were normalized by GAPDH mRNA. <svg height="15.3614pt" id="M3" style="vertical-align:-3.833899pt" version="1.1" viewbox="-0.0498162 -11.5275 55.736 15.3614" width="55.736pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M457 455H370L403 612H360L326 455H218L252 612H210L175 455H77L68 394H161L132 257H31L20 196H117L77 19H120L159 196H268L230 19H272L311 196H402L412 257H325L355 394H447L457 455ZM313 394L283 257H175L204 394H313Z"></path></g><g transform="matrix(.013,0,0,-0.013,4.946,-1.651)"><path d="M570 304C570 398 525 448 414 448C385 448 343 445 312 434L329 511L321 518C297 504 262 482 244 460L233 411C195 397 159 381 128 358L135 332C160 347 189 360 224 373L111 -147C97 -210 84 -218 17 -231L13 -257L254 -247L259 -218L233 -216C183 -212 177 -202 189 -142L218 -1C238 -10 266 -12 283 -12C351 3 429 48 483 105C543 168 570 242 570 304ZM482 289C482 161 380 33 304 33C278 33 248 51 233 69L303 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(c) Flow cytometry of cellular ROS in NTERA-2 and NCCIT cells after treatment with 20 <i>μ</i>M UA for 24 h. Image presentation has shown cells with cellular ROS induction. (d) Flow cytometry of mitochondrial ROS upon 20 <i>μ</i>M UA treatment for 24 h in NTERA-2 and NCCIT cells. The graphical image has shown cells with ROS induction in mitochondria.</div>

Journal of Oncology

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Figure 2

Figure 2: Antitumor Effects of Natural Bioactive Ursolic Acid in Embryonic Cancer Stem Cells 