Antitumor Effects of Natural Bioactive Ursolic Acid in Embryonic Cancer Stem Cells

<div>UA induces G0/G1 cell cycle arrest. (a) Flow cytometry using PI showing cell cycle distribution in NTERA-2 and NCCIT cells after 24 h treatment with 20 <i>μ</i>M UA. (b) Immunoblotting analysis of NTERA-2 and NCCIT cells after 24 h treatment with 10 or 20 <i>μ</i>M UA showing expression of cyclin D1, cyclin E CDK4, p21, and p27 proteins. Exposure rates were measured by densitometry and are standardized in <i>β</i>-actin levels. Data were obtained in triplicate. (c) RT-qPCR showing genetic expression of a cell cycle test. References representing CDK4, CDKN1B, CCNE1, CDKN1A, and CCND1 mRNA were displayed; Cp values were normalized to GAPDH mRNA. 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Journal of Oncology

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Figure 4

Figure 4: Antitumor Effects of Natural Bioactive Ursolic Acid in Embryonic Cancer Stem Cells 