Downregulation of hsa-miR-30b-3p Inhibits the Oncogenicity of Lung Adenocarcinoma by Targeting the METTL7B Gene

Figure 3

miR-30b-3p targets the METTL7B gene. (a) Prediction of METTL7B as a target of miR-30b-3p by bioinformatics analysis. (b) Validation of the targeting of METTL7B by miR-30b-3p by a dual luciferase assay. (c) Expression and correlation of miR-30b-3p and METTL7B in LUAD tissues were determined and analyzed by qRT-PCR. (d) qRT-PCR analysis was used to determine the expression level of METTL7B in 30 LUAD tissues and normal lung tissues. (e) ENCORI platform-based analysis of METTL7B expression in a large sample of LUAD tissues. (f) qRT-PCR analysis of METTL7 expression in the LUAD cell lines A549, PC-9, and H1975 and the human bronchial epithelial cell line BEAS-2B. (g) Detection of METTL7 protein expression in miR-30b-3p-overexpressing LUAN cells by Western blot analysis. .

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