MicroRNA-323a-3p Negatively Regulates NEK6 in Colon Adenocarcinoma Cells

Figure 3

miR-323a-3p inhibits cell proliferation by negatively regulating NEK6. (a, b) After cells were transfected with the pCDH-NEK6 plasmid, the expression level of NEK6 was evaluated via RT-PCR (a) and western blotting (b) assays. (c–h) After the miR-323a-3p or mimic NC was cotransfected into HCT-8 and SW620 cells with the pCDH-NEK6 plasmid or pCDH vector, RT-PCR (c, d) and western blotting (e) assays were performed to assess the expression levels of miR-323a-3p and NEK6. MTT (f) and EdU (g) assays were employed to evaluate cell proliferation, and PI staining was performed for the cell cycle assay (h). PI: propidium iodide; ns: not significant, , , , and .

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