MicroRNA-323a-3p Negatively Regulates NEK6 in Colon Adenocarcinoma Cells

Table 2

The primers for construction of plasmids.

Name of plasmids	Name of primers	Sequence (5′-3′)
Wild-type NEK6 3′ UTR	3′UTR WT-F	GAGCTCGCTAGCCTCGAGCCGTGCCTTATCAAAGCCAG
	3′UTR WT-R	GCATGCCTGCAGGTCGACGCAGCAGGTGTCAGGAATC
Mutant NEK6 3′ UTR	3′UTR MUT-F	TGGATTGATTACAGAATCTTTAGGGTAATTC
	3′UTR MUT-R	AAGATTCTGTAATCAATCCAGATACAGGGGGC
pCDH-NEK6	pCDH-Nflag-F	CTAGAGCTAGCGAATTCGCCACCATGGACTACAAAGACGATGACGAC
	Flag-NEK6-F	ACAAAGACGATGACGACAAGATGCCCAGGAGAGAAGTTTG
	pCDH-NEK6-R	CTCAGCGGCCGCGGATCCGGTGCTGGACATCCAGATG

F: forward primer; R: reverse primer.