circCCT3 Enhances Invasion and Epithelial-Mesenchymal Transition (EMT) of Non-Small-Cell Lung Cancer (NSCLC) via the miR-107/Wnt/FGF7 Axis

Figure 2

The miR-107 mimic rescued circCCT3-induced invasion and EMT of NSCLC cell. (a) StarBase 2.0 predicted miR-107 as the potential target for circCCT3. (b) RT-qPCR showed miR-107 and circCCT3 levels in A549 cells transiently transfected with the NC mimic, miR-107 mimic, NC inhibitor, and miR-107 inhibitor. , miR-107 mimic vs NC mimic group; ^###, circTTC3 + miR-107 mimic vs circTTC3 group. (c) RT-qPCR showed miR-107 levels in A549 cells transiently transfected with pcDNA3.1 (EV) and pcDNA3.1-circCCT3 (circCCT3) and pcDNA3.1-circCCT3 plus miR-107 mimic. , circTT3C vs EV group; ^###, circTTC3 + miR-107 mimic vs circTTC3 group. (d, e) Cell invasion was examined in A549 cells transiently transfected with pcDNA3.1 (EV) and pcDNA3.1-circCCT3 (circCCT3) and pcDNA3.1-circCCT3 plus miR-107 mimic. , miR-107 mimic vs NC mimic group; ^##, circTTC3 + miR-107 mimic vs circTTC3 group. (f, g) E-cadherin and vimentin levels were measured by RT-qPCR in A549 cells transiently transfected with pcDNA3.1 (EV) and pcDNA3.1-circCCT3 (circCCT3) and pcDNA3.1-circCCT3 plus miR-107 mimic. , miR-107 mimic vs NC mimic group; ^##, ^###, circTTC3 + miR-107 mimic vs circTTC3 group.

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