Research Article
Inhibition of Angiogenesis by MiR-524-5p through Suppression of AKT and ERK Activation by Targeting CXCR7 in Colon Cancer Cells
Figure 5
MiR-524-5p regulates angiogenesis via AKT/ERK signaling in HT-29 cells. (a) Cell proliferation was measured by the CCK8 assay in HT-29 cells after cotransfection. The release of CXCL11 (b), CXCL12 (c), and VEGF (d) in HT-29 cells after cotransfection. (e) Representative images of migration and tube formation assays. (f) Quantification of migration ability. (g) Quantification of angiogenic ability. (h) Protein levels of CXCR7, AKT, p-AKT, ERK, p-ERK, VEGF, and PDGF in HT-29 cells after cotransfection.
(a) |
(b) |
(c) |
(d) |
(e) |
(f) |
(g) |
(h) |