Inhibition of Angiogenesis by MiR-524-5p through Suppression of AKT and ERK Activation by Targeting CXCR7 in Colon Cancer Cells

Figure 6

MiR-524-5p regulates angiogenesis by AKT/ERK signaling in Caco-2 cells. (a) Cell proliferation is measured by the CCK8 assay in Caco-2 cells after cotransfection. The release of CXCL11 (b), CXCL12 (c), and VEGF (d) in Caco-2 cells after cotransfection. (e) Representative images of migration and tube formation assays. (f) Quantification of migration ability. (g) Quantification of angiogenic ability. (h) Protein levels of CXCR7, AKT, p-AKT, ERK, p-ERK, VEGF, and PDGF in Caco-2 cells after cotransfection.

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