CircMAT2B Induced by TEAD1 Aggravates the Warburg Effect and Tumorigenesis of Oral Squamous Cell Carcinoma through the miR-942-5p/HSPD1 Axis

Figure 2

CircMAT2B knockdown inhibits OSCC tumorigenesis and the Warburg effect. (a) and (b) OSCC cells Cal-27 and HSC-6 were infected with Sh-NC, Sh-circMAT2B#1, and Sh-circMAT2B#2, respectively. CircMAT2B (a) and MAT2B (b) expression was measured by qRT-PCR. (c) and (d) CircMAT2B knockdown or normal control Cal-27 or HSC-6 cells were subjected to EdU assay for proliferation ability detection (c) and analysis (d). (e) and (f) transwell migration assay was performed to evaluate migration level of circMAT2B knockdown or normal control Cal-27 or HSC-6 cells (e) and (f). (g) and (h) transwell invasion assay was performed to evaluate the invasion of circMAT2B knockdown or normal control Cal-27 or HSC-6 cells (g) and (h). (i)–(k) warburg effect relative glucose uptake (i), lactate production (j), and ATP levels (k) were detected. Each experiment was performed three times. Data are presented as mean ± SD. , , .

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