Research Article

CircMAT2B Induced by TEAD1 Aggravates the Warburg Effect and Tumorigenesis of Oral Squamous Cell Carcinoma through the miR-942-5p/HSPD1 Axis

Figure 6

MiR-942-5p directly targets HSPD1. (a): potential mRNA targets for HSPD1 predicted by bioinformatics analysis. (b) and (c): biotinylated NC probes and miR-942-5p probes were transfected into Cal-27 (b) and HSC-6 (c) cells and the putative miRNA target expression in pull-down bounds was measured by qRT-PCR. D-E: NC mimic or miR-942-5p mimic was infected into Cal-27 (d) or HSC-6 (e) cells and the relative expression of putative mRNA targets was measured by qRT-PCR. (f): bioinformatic prediction of the HSPD1-miR-942-5p binding. (g) and (h): A luciferase reporter gene assay was conducted to confirm the interaction between HSPD1 and miR-942-5p. Each experiment was performed three times. Data are presented as mean ± SD. , , .
(a)
(b)
(c)
(d)
(e)
(f)
(g)
(h)