Ultraviolet Radiation Promoted Hypoxia-Induced Apoptosis in HL-60 Human Promyelocytic Leukemia Cell Line

Figure 4

UV LED irradiation and hypoxia reduced Bcl-2 protein level and increased Bax, caspase 3, cleaved-caspase 3 and caspase 9 protein levels. (a) Western blot analysis of protein levels of Bax and Bcl-2. (b) Western blot analysis of protein levels of caspase 3, cleaved-caspase 3 and caspase 9. The relative protein levels of Bax (c), Bcl-2 (d), caspase 3(e), cleaved-caspase 3 (f) and caspase 9 (g) in the cells exposed to UV LED and CoCl₂ in different groups were compared with those of the untreated cells (set as 1). (h) The Bcl-2/Bax protein ratio in different groups. ^∗ vs. the control.

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