UHRF1 Induces Metastasis in Thyroid Cancer

Figure 4

UHRF1 combined directly with c-Jun/AP-1 in the nucleus. BCPAP cells were transfected according to the panel labels. The co-IP assay was performed using either an anti-Flag antibody to pull down Flag-tagged c-Jun proteins (a) or an anti-His antibody against UHRF1-His protein (b). BCPAP and 293T cells were cotransfected with UHRF1-His and c-Jun-Flag. Immunofluorescence staining was performed to visualize the colocalization of UHRF1 (green) and c-Jun (red). Nuclear DNA was stained with DAPI (blue) (c, d). The scale bar represents 100 μm.

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