HOTAIRM1 Maintained the Malignant Phenotype of tMSCs Transformed by GSCs via E2F7 by Binding to FUS

Figure 1

HOTAIRM1 is upregulated in tMSCs and promotes the proliferation, migration, and invasion of tMSCs. (a) qRT-PCR analysis of HOTAIRM1 expression in normal mesenchymal stromal/stem cells (MSCs) and transformed mesenchymal stromal/stem cells (tMSCs). (b) qRT-PCR analysis of HOTAIRM1 expression in tMSC1 and tMSC3 cells transfected with control (sh-NC), sh-HOTAIRM1-1, and sh-HOTAIRM1-2. (c) qRT-PCR analysis of HOTAIRM1 expression in tMSC1 and tMSC3 cells transfected with empty vector (Vector) and pcDNA-HOTAIRM1. (d, e) Colony formation assays were performed to detect the proliferation of tMSC1 and tMSC3 cells with different expression levels of HOTAIRM1. (f–g) EdU assays were used to determine the proliferation of tMSC1 and tMSC3 cells with different expression levels of HOTAIRM1. (h, i) Transwell assays were performed to detect changes in migratory abilities of tMSC1 and tMSC3 cells with different expression levels of HOTAIRM1. (j, k) Transwell assays were performed to detect changes in invasion abilities of tMSC1 and tMSC3 cells with different expression levels of HOTAIRM1. means .

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