HOTAIRM1 Maintained the Malignant Phenotype of tMSCs Transformed by GSCs via E2F7 by Binding to FUS

Figure 3

HOTAIRM1 can bind to FUS. (a, b) qRT-PCR was used to verify the subcellular localization of HOTAIRM1 in tMSC1 and tMSC3 cells. Change in E2Fs after HOTAIRM1 knockdown in tMSC1 and tMSC3 cells. (c) In tMSC1, RIP experiments were performed for the indicated RBPs, and coprecipitated RNA was subjected to qRT-PCR for HOTAIRM1. (d) RIP experiments were performed for FUS, and the coprecipitated RNA was subjected to qRT-PCR for HOTAIRM1 in tMSC3. (e, f) qRT-qPCR and Western blotting were performed to detect FUS mRNA and protein levels after HOTAIRM1 knockdown in tMSC1 and tMSC3 cells. means .

(a)

(b)

(c)

(d)

(e)

(f)