HOTAIRM1 Maintained the Malignant Phenotype of tMSCs Transformed by GSCs via E2F7 by Binding to FUS

Figure 4

HOTAIRM1 promotes the proliferation, migration, and invasion of tMSCs by regulating E2F7. (a, b) In tMSC1 and tMSC3, RIP experiments were performed for FUS, and coprecipitated RNA was subjected to qRT-PCR for E2Fs. (c, d) Colony formation assays were performed to evaluate the proliferation of sh-E2F7- and pcDNA-HOTAIRM1-cotransfected tMSC1 and tMSC3 cells. (e, f) EdU assays were used to determine the proliferation of sh-E2F7 and pcDNA-HOTAIRM1-cotransfected tMSC1 and tMSC3 cells. (g, h) Transwell assays were performed to detect changes in migration of sh-E2F7 and pcDNA-HOTAIRM1-cotransfected tMSC1 and tMSC3 cells. (i, j) Transwell assays were performed to detect changes in invasion of sh-E2F7 and pcDNA-HOTAIRM1-cotransfected tMSC1 and tMSC3 cells. means .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)