HOTAIRM1 Maintained the Malignant Phenotype of tMSCs Transformed by GSCs via E2F7 by Binding to FUS

Figure 5

HOTAIRM1 functions as an oncogene by regulating E2F7 by binding to FUS. (a–d) qRT-PCR and Western blotting were performed to detect the expression of E2F7 in pcDNA-HOTAIRM1 and sh-FUS-cotransfected tMSC1 and tMSC3 cells. (e, f) Colony formation assays were performed to evaluate the proliferation of pcDNA-HOTAIRM1 and sh-FUS-cotransfected tMSC1 and tMSC3 cells. (g, h) EdU assays were used to determine the proliferation of pcDNA-HOTAIRM1- and sh-FUS-cotransfected tMSC1 and tMSC3 cells. (i, j) Transwell assays were performed to detect changes in migration of pcDNA-HOTAIRM1- and sh-FUS-cotransfected tMSC1 and tMSC3 cells. (k, l) Transwell assays were performed to detect changes in invasion of pcDNA-HOTAIRM1- and sh-FUS-cotransfected tMSC1 and tMSC3 cells. means .

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