Identification of KIF21B as a Biomarker for Colorectal Cancer and Associated with Poor Prognosis

Figure 6

KIF21B was upregulated in colorectal cancer cells and promotes survival, increases migration and invasion. (a) KIF21B mRNA expression in CRC cell lines was measured by RT-qPCR. (b) Western blotting assay was used to analyze KIF21B protein expression in CRC cell lines. (c) HT29 and HCT116 cell lines were transfected with shNC and shKIF21B, respectively. The expression of KIF21B was measured by Western blotting. (d) KIF21B-knockdown HCT116 and HT29 cell lines were cultured as the indicated time (0 h, 24 h, 48 h, 72 h, and 96 h), then the cell proliferation was measured by CCK8 assay. (e) Cell apoptosis in KIF21B deficiency HCT116 and HT29 cell lines was measured by flow cytometry. (f, g) Cell migration and invasion were measured by transwell assay without or with matrigel.

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