Tetrahydrocurcumin Chemosensitizes Breast Cancer to Albumin-Bound Paclitaxel by Enhancing SPARC Expression through Demethylation

Table 1

Information for primer and sequence.

Primer	Location
SPARC		Forward: 5-ATCTTCCCTGTACACTGGCAGTTC-3
		Reverse: 5-CTCGGTGTGGGAGAGGTACC-3
GADPH		Forward: 5-CCTGGACTTCGAGCAAGAGAT-3
		Reverse: 5-GCCGATCCACACGGAGTACT-3
#1 siRNA SPARC	306	GCAUCAAGCAGAAGGAUAUTTAUAUCCUUCUGCUUGAUGCTT
#2 siRNA SPARC	448	GCAGAGGUGACUGAGGUAUTTAUACCUCAGUCACCUCUGCTT
#3 siRNA SPARC	1073	GCGAGCUGGAUGAGAACAATTUUGUUCUCAUCCAGCUCGCTT
Negative control (scramble)		UGACCUCAUCUACAUGGUUTTAACCAUGUAGUUGAGGUCATT
mSPARC primer	112 bp	Forward: 5-GAGAGCGCGTTTTGTTTGTC-3
		Reverse: 5-AACGACGTAAACGAAAATATCG-3
uSPARC primer	132 bp	Forward: 5-TTTTTTAGATTGTTTGGAGAGTG-3
		Reverse: 5-AACTAACAACATAAACAAAAATATC-3