Overexpressed lncRNA LINC00893 Suppresses Progression of Colon Cancer by Binding with miR-146b-3p to Upregulate PRSS8

Figure 3

LINC00893 binds with miR-146b-3p in colon cancer cells. (a) The targeted sequence of LINC00893 to miR-146b-3p by LncBase online tool. (b) The RNA pull-down assay was carried out to evaluate the binding between LINC00893 and miR-146b-3p in HCT116 and SW620 cells. (c) Overexpression efficiency of miR-146b-3p in HCT116 and SW620 cells was assessed by RT-qPCR. (d) The dual-luciferase assays were performed to investigate the binding between LINC00893 and miR-146b-3p. (e) Transwell assay was used to detect the invasion of HCT116 and SW620 cells transfected with LINC00893 OE, , and empty vector. (f) MTT assay was used to detect the cell viability in four groups. (g) EdU assay showed the cell proliferation in transfected cells. (h) TUNEL assay was performed to evaluate the apoptotic ability of transfected cells in four groups. (i, j) Cell cycle arrest of (i) HCT116 and (j) SW620 cells after transfection with LINC00893 overexpressing vector and miR-146b-3p mimics was assessed by flow cytometry analysis. , , ^#, ^##.

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