Overexpressed lncRNA LINC00893 Suppresses Progression of Colon Cancer by Binding with miR-146b-3p to Upregulate PRSS8

Figure 4

PRSS8 is a target of miR-146b-3p and their functions in colon cancer cells. (a) The binding site between miR-146b-3p and PRSS8 was predicted by Targetscan. (b) Western blot and RT-qPCR analyses were used to analyze the protein and mRNA expression of PRSS8 in si-PRSS8 (1#, 2#, 3#), si-NC, and control groups in HCT116 and SW620 cells. (c) Luciferase reporter assay was performed to investigate the binding between miR-146b-3p and PRSS8 in HCT116 colon cancer cells. (d) Transwell assay showed invasion of HCT116 and SW620 cells after transfections with miR-146b-4p inhibitors, , and . (e) MTT assay represented the cell viability of four groups in transfected cells. (f) EdU assay demonstrated the cell proliferation in transfected cells. (g) TUNEL assay was performed to evaluate the apoptotic ability of four groups in transfected cells. (h, i) Cell cycle arrest of (h) HCT116 and (i) SW620 cells after transfection with si-PRSS8 and miR-146b-3p inhibitor was assessed by flow cytometry analysis. (j) PRSS8 expression in colon cancer cells transfected with LINC00893 OE. (k) PRSS8 expression in colon cancer cells transfected with miR-146b-3p mimics. , , , ^#, ^##, ^###.

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