Periplocin Induces Apoptosis of Pancreatic Cancer Cells through Autophagy via the AMPK/mTOR Pathway

Figure 2

Periplocin induces autophagy in both CFPAC1 and PANC-1 cells. (a) Cluster heat map of autophagy genes. Downregulated genes are shown in blue, and upregulated genes are shown in red. (b) LC3B Immunofluorescence when CFPAC1 and PANC-1 cells were treated with Periplocin (125 nM, 250 nM) or an equal volume of DMEM media for 24 h. (c, d) The effects of Periplocin on the expression of p62 and LC3BII/I proteins in CFPAC1 and PANC-1 cells, as determined by Western blotting analysis. (e) Statistical analysis of the mean fluorescence intensity of the LC3 protein. (f) Western blot quantitative statistical analysis of the LC3B and P62 proteins. The data represent the mean ± SE, with , , compared between two groups (n = 3).

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